Composite

Part:BBa_K3002211:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + GLE_Mut-PETase + cCA_MHETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal NheI site found at 2665
    Illegal NheI site found at 5579
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
    Illegal NotI site found at 6992
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal BglII site found at 7902
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3238
    Illegal NgoMIV site found at 3265
    Illegal NgoMIV site found at 4916
    Illegal NgoMIV site found at 6590
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002107 (GLE_Mutant PETase) and BBa_K3002113 (cCA_wildtype MHETase).

References